Our rodent treadmill is equipped with a grid at the rear end, capable of delivering direct-current shocks.
Protocol: With the treadmill at the horizontal position, the tests are preceded by a five-minute period at a low speed (10m/min), considered necessary for the adaptation of the animals. Subsequently, the rats are exercised to the point of exhaustion, defined as the point when the rat can no longer keep pace with the speed of the belt and remains on the grid, despite electric shocks.
End-point: The total distance covered by the animal is recorded.